ARG23149
anti-Fc epsilon RI antibody [9E1]
anti-Fc epsilon RI antibody [9E1] for ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [9E1] recognizes Fc epsilon RI Mouse anti Human Fc epsilon R1 alpha antibody, clone 9E1 recognizes the human high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response. Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits. Clone 9E1 specifically recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain.Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, ICC/IF, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 9E1 |
| Isotype | IgG2b |
| Target Name | Fc epsilon RI |
| Antigen Species | Human |
| Conjugation | Un-conjugated |
| Alternate Names | High affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; FcERI; FCE1A; IgE Fc receptor subunit alpha |
Application Instructions
| Application Suggestion |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P12319 Human High affinity immunoglobulin epsilon receptor subunit alpha |
|---|---|
| Gene Symbol | FCER1A |
| Gene Full Name | Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide |
| Background | The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011] |
| Function | Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. [UniProt] |
| Calculated MW | 30 kDa |
Images (1) Click the Picture to Zoom In
-
ARG23149 anti-Fc epsilon RI antibody [9E1] FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG23149 anti-Fc epsilon RI antibody [9E1], showing staining of eosinophils.
