ARG55898
anti-Fatty Acid Synthase antibody
anti-Fatty Acid Synthase antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat,Hamster
Overview
| Product Description | Mouse Monoclonal antibody recognizes Fatty Acid Synthase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Hm |
| Predict Reactivity | Mk |
| Tested Application | ICC/IF, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Target Name | Fatty Acid Synthase |
| Antigen Species | Human |
| Immunogen | Purified recombinant Human Fatty Acid Synthase fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.3.1.85; EC 1.3.1.39; Fatty acid synthase; EC 2.3.1.41; FAS; EC 4.2.1.59; OA-519; EC 2.3.1.38; EC 2.3.1.39; EC 3.1.2.14; EC 1.1.1.100; SDR27X1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.03% Proclin-300 and 50% Glycerol |
| Preservative | 0.03% Proclin-300 |
| Stabilizer | 50% Glycerol |
| Concentration | 4.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FASN |
| Gene Full Name | fatty acid synthase |
| Background | The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008] |
| Function | Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. [UniProt] |
| Cellular Localization | Cytoplasm; Melanosome |
| Calculated MW | 273 kDa |
Images (3) Click the Picture to Zoom In
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ARG55898 anti-Fatty Acid Synthase antibody WB image
Western blot: 30 µg of HeLa and 293T cell lysates stained with ARG55898 anti-Fatty Acid Synthase antibody at 1:1000 dilution.
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ARG55898 anti-Fatty Acid Synthase antibody ICC/IF image
Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG55898 anti-Fatty Acid Synthase antibody at 1:400 dilution.
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ARG55898 anti-Fatty Acid Synthase antibody IP image
Immunoprecipitation: CHO-K1 cell lysates were immunoprecipitated and stained with ARG55898 anti-Fatty Acid Synthase antibody.
Customer's Feedback
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Review for anti-Fatty Acid Synthase antibody
Application：WB
Sample：3T3
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
