ARG54560
anti-Factor V Heavy chain antibody [B10]
anti-Factor V Heavy chain antibody [B10] for ELISA and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Mouse Monoclonal antibody [B10] recognizes Factor V Heavy chain |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | This antibody reacts with the activation C peptide (mw ~150,000) in the heavy chain of thrombin-cleaved human Factor V. KD = 1.15 x 10-10 mol/L. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B10 |
| Isotype | IgG1 |
| Target Name | Factor V Heavy chain |
| Antigen Species | Human |
| Immunogen | Purified human Factor V. |
| Conjugation | Un-conjugated |
| Alternate Names | FVL; Activated protein C cofactor; PCCF; Coagulation factor V; THPH2; Proaccelerin, labile factor; RPRGL1 |
Application Instructions
| Application Note | This antibody may be used in ELISA, immunohistochemistry, and autoradiography. Other applications are under investigation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Protein G-purified |
| Buffer | PBS (pH 7.4) |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | F5 |
| Gene Full Name | coagulation factor V (proaccelerin, labile factor) |
| Background | This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008] |
| Function | Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 252 kDa |
| PTM | Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus). Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity. Activated protein C inactivates factor V and factor Va by proteolytic degradation. Phosphorylated by FAM20C in the extracellular medium. |
