ARG70022

anti-Factor IX antibody

anti-Factor IX antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Goat Polyclonal antibody recognizes Factor IX
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Factor IX
Antigen Species Human
Immunogen Human Factor IX purified from plasma.
Conjugation Un-conjugated
Alternate Names Coagulation factor IX; HEMB; FIX; PTC; Plasma thromboplastin component; F9 p22; THPH8; EC 3.4.21.22; P19; Christmas factor

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 10 mM HEPES (pH 7.4), 150 mM NaCl and 50% (v/v) Glycerol.
Stabilizer 50% (v/v) Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2158 Human F9

Swiss-port # P00740 Human Coagulation factor IX

Gene Symbol F9
Gene Full Name coagulation factor IX
Background This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Sep 2015]
Function Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. [UniProt]
Cellular Localization Secreted [UniProt]
Calculated MW 52 kDa
PTM Activated by factor XIa, which excises the activation peptide (PubMed:9169594, PubMed:1730085). The propeptide can also be removed by snake venom protease (PubMed:20004170, PubMed:20080729).

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

Predominantly O-glucosylated at Ser-99 by POGLUT1 in vitro. Xylosylation at this site is minor. [UniProt]