ARG64013
anti-FXR1 antibody
anti-FXR1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse
Gene Regulation antibody; Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes FXR1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat, Dog |
| Tested Application | FACS, ICC/IF, WB |
| Specificity | This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FXR1 |
| Antigen Species | Human |
| Immunogen | C-RIEGDNENKLPRED |
| Conjugation | Un-conjugated |
| Alternate Names | hFXR1p; FXR1P; Fragile X mental retardation syndrome-related protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P51114 Human Fragile X mental retardation syndrome-related protein 1 Swiss-port # Q61584 Mouse Fragile X mental retardation syndrome-related protein 1 |
|---|---|
| Background | The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody; Immune System antibody |
| Calculated MW | 70 kDa |
| PTM | Arg-445 is dimethylated, probably to asymmetric dimethylarginine. |
Images (4) Click the Picture to Zoom In
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ARG64013 anti-FXR1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG64013 anti-FXR1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64013 anti-FXR1 antibody WB image
Western blot: 35 µg of NIH/3T3 cell lysate (in RIPA buffer) stained with ARG64013 anti-FXR1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG64013 anti-FXR1 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG64013 anti-FXR1 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG64013 anti-FXR1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG64013 anti-FXR1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
