ARG63656
anti-FTCD / 58K Golgi protein antibody
anti-FTCD / 58K Golgi protein antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Pig
Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes FTCD / 58K Golgi protein |
|---|---|
| Tested Reactivity | Hu, Ms, Pig |
| Predict Reactivity | Cow, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Specificity | Variants (NP_006648.1; NP_996848.1) encode the same protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FTCD / 58K Golgi protein |
| Antigen Species | Human |
| Immunogen | CLREQGRGKDQPGRL |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.1.2.5; LCHC1; Formiminotetrahydrofolate cyclodeaminase; Formimidoyltransferase-cyclodeaminase; Formiminotransferase-cyclodeaminase; Glutamate formiminotransferase; Glutamate formyltransferase; FTCD; EC 4.3.1.4 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Human, Mouse and Pig liver. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Dec 2009] |
| Research Area | Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 59 kDa |
Images (4) Click the Picture to Zoom In
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ARG63656 anti-FTCD / 58K Golgi protein antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63656 anti-FTCD / 58K Golgi protein antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63656 anti-FTCD / 58K Golgi protein antibody WB image
Western blot: 35 µg of Human liver, Mouse liver and Pig liver lysates stained with ARG63656 anti-FTCD / 58K Golgi protein antibody at 0.1 µg/ml (Human and Mouse data) or 0.3 µg/ml (Pig data) and incubated at RT for 1 hour.
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ARG63656 anti-FTCD / 58K Golgi protein antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.5% Triton. Cells were stained with ARG63656 anti-FTCD / 58K Golgi protein antibody at 10 µg/ml dilution for 1 hour (blue line) or Goat IgG isotype control antibody (black line), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
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ARG63656 anti-FTCD / 58K Golgi protein antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63656 anti-FTCD / 58K Golgi protein antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
