ARG66269
anti-FRS2 phospho (Tyr436) antibody
anti-FRS2 phospho (Tyr436) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes FRS2 phospho (Tyr436) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | The antibody detects endogenous levels of FRS2 only when phosphorylated at tyrosine 436. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FRS2 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated phospho-specific peptide around Tyr436 (LNY(p)IQ) of Human FRS2. |
| Conjugation | Un-conjugated |
| Alternate Names | Fibroblast growth factor receptor substrate 2; FRS2A; SNT1; SNT-1; SNT; FRS2alpha; FGFR substrate 2; Suc1-associated neurotrophic factor target 1; FGFR-signaling adaptor SNT |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with phospho-specific peptide and the non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. |
| Buffer | PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8WU20 Human Fibroblast growth factor receptor substrate 2 |
|---|---|
| Gene Symbol | FRS2 |
| Gene Full Name | fibroblast growth factor receptor substrate 2 |
| Function | Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1. [UniProt] |
| Calculated MW | 57 kDa |
| PTM | Phosphorylated by ULK2 in vitro (By similarity). Phosphorylated on tyrosine residues upon stimulation by NGF or FGF2. Phosphorylated on tyrosine residues by activated ALK and FGFR1. Phosphorylated on tyrosine residues upon activation of FGFR2 and FGFR3. Phosphorylated on threonine residues by MAP kinases; this inhibits tyrosine phosphorylation, and thereby down-regulates FRS2-mediated activation of MAP kinases. Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination (By similarity). [UniProt] |
Images (2) Click the Picture to Zoom In
-
ARG66269 anti-FRS2 phospho (Tyr436) antibody WB image
Western blot: 1, 2) HuVEC and 3) JK cells. 1) Treated with antigen-specific peptide; 2, 3) Untreated. The blots were stained with ARG66269 anti-FRS2 phospho (Tyr436) antibody.
-
ARG66269 anti-FRS2 phospho (Tyr436) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG66269 anti-FRS2 phospho (Tyr436) antibody (left) or the same antibody pre-incubated with blocking peptide (right).
