ARG54203

anti-FOXP3 antibody [3G3] (PE)

anti-FOXP3 antibody [3G3] (PE) for Flow cytometry and Human,Mouse

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Regulatory T cells Study antibody

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [3G3] recognizes FOXP3
Tested Reactivity Hu, Ms
Tested Application FACS
Specificity The mouse monoclonal antibody 3G3 recognizes Nterminal region of FoxP3, a 4755 kDa transcription factor, which is the master regulator in the development and function of regulatory T cells.
Host Mouse
Clonality Monoclonal
Clone 3G3
Isotype IgG1
Target Name FOXP3
Antigen Species Mouse
Immunogen Full-length His-tagged recombinant murine FoxP3_x000D_
Conjugation PE
Alternate Names FOXP3; Forkhead Box P3; DIETER; XPID; AIID; PIDX; IPEX; JM2; Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked; Forkhead Box Protein P3; SCURFIN; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; FOXP3delta7; Scurfin

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20371 Mouse FOXP3

GeneID: 50943 Human FOXP3

Swiss-port # Q99JB6 Mouse Forkhead box protein P3

Swiss-port # Q9BZS1 Human Forkhead box protein P3

Gene Symbol FOXP3
Gene Full Name forkhead box P3
Background The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Function Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). [UniProt]

Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. [UniProt]
Cellular Localization Cytoplasm, Nucleus. [UniProt]
Highlight Related products:
FOXP3 antibodies; FOXP3 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Regulatory T cells Study antibody
Calculated MW 47 kDa
PTM Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG54203 anti-FOXP3 antibody [3G3] (PE) FACS image

    Flow Cytometry: Human peripheral blood cells (gated on CD4+ cells) stained with ARG54203 anti-FOXP3 antibody [3G3] (PE) and anti-CD25 antibody (APC).

Clone References

Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development.

Gavin MA et al.
Proc Natl Acad Sci U S A.,  (2006)

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Protein phosphatase 5 is a major component of glucocorticoid receptor.hsp90 complexes with properties of an FK506-binding immunophilin.

IP / Rabbit

Silverstein AM et al.
J Biol Chem.,  (1997)

publication_link

 

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Folding of the glucocorticoid receptor by the reconstituted Hsp90-based chaperone machinery. The initial hsp90.p60.hsp70-dependent step is sufficient for creating the steroid binding conformation.

IP / Mouse

Dittmar KD et al.
J Biol Chem.,  (1997)

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hr_line

Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons.

WB / Rat

Benson DL et al.
J Neurocytol.,  (1996)

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The basic helix-loop-helix/PAS factor Sim is associated with hsp90. Implications for regulation by interaction with partner factors.

IP / D. melanogaster

McGuire J et al.
J Biol Chem.,  (1995)

publication_link

 

hr_line

All of the factors required for assembly of the glucocorticoid receptor into a functional heterocomplex with heat shock protein 90 are preassociated in a self-sufficient protein folding structure, a foldosome.

WB / Mouse

Hutchison KA et al.
J Biol Chem.,  (1994)

publication_link

 

hr_line

The 23-kDa acidic protein in reticulocyte lysate is the weakly bound component of the hsp foldosome that is required for assembly of the glucocorticoid receptor into a functional heterocomplex with hsp90.

WB / Rabbit

Hutchison KA et al.
J Biol Chem.,  (1995)

publication_link

 

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