ARG58097

anti-FOXO3A antibody

anti-FOXO3A antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes FOXO3A
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FOXO3A
Antigen Species Mouse
Immunogen Recombinant fusion protein corresponding to aa. 394-672 of Mouse FOXO3A (NP_062714.1).
Conjugation Un-conjugated
Alternate Names FKHRL1; Fkhr2; 2010203A17Rik; Forkhead box protein O3; 1110048B16Rik; C76856; Foxo3a

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 90 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2309 Human FOXO3

GeneID: 56484 Mouse FOXO3

Swiss-port # O43524 Human Forkhead box protein O3

Swiss-port # Q9WVH4 Mouse Forkhead box protein O3

Gene Symbol Foxo3
Gene Full Name forkhead box O3
Function Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (By similarity). [UniProt]
Calculated MW 71 kDa

Images (6) Click the Picture to Zoom In

  • ARG58097 anti-FOXO3A antibody WB image

    Western blot: Mouse kidney stained with ARG58097 anti-FOXO3A antibody.

  • ARG58097 anti-FOXO3A antibody IHC-P image

    Immunohistochemistry: Human colon stained with ARG58097 anti-FOXO3A antibody at 1:50 dilution.

  • ARG58097 anti-FOXO3A antibody WB image

    Western blot: Jurkat, 293T and PC-3 stained with ARG58097 anti-FOXO3A antibody at 1:1000 dilution.

  • ARG58097 anti-FOXO3A antibody ICC/IF image

    Immunofluorescence: C6 stained with ARG58097 anti-FOXO3A antibody at 1:100 dilution.

  • ARG58097 anti-FOXO3A antibody IHC-P image

    Immunohistochemistry: Mouse kidney stained with ARG58097 anti-FOXO3A antibody at 1:50 dilution.

  • ARG58097 anti-FOXO3A antibody ICC/IF image

    Immunofluorescence: NIH/3T3 stained with ARG58097 anti-FOXO3A antibody at 1:100 dilution.