ARG53038

anti-FOXA1 / HNF3A antibody [SP88]

anti-FOXA1 / HNF3A antibody [SP88] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Gene Regulation antibody

Overview

Product Description Rabbit Monoclonal antibody [SP88] recognizes FOXA1 / HNF3A
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P
Host Rabbit
Clonality Monoclonal
Clone SP88
Isotype IgG
Target Name FOXA1 / HNF3A
Antigen Species Human
Immunogen Synthetic peptide derived from near C-terminus of human FOXA1 protein.
Conjugation Un-conjugated
Alternate Names TCF3A; Forkhead box protein A1; Hepatocyte nuclear factor 3-alpha; TCF-3A; HNF-3A; HNF-3-alpha; HNF3A; Transcription factor 3A

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-Dependent
ICC/IFAssay-Dependent
IHC-P1:100
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Prostate

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3169 Human FOXA1

Swiss-port # P55317 Human Hepatocyte nuclear factor 3-alpha

Background This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.
Cellular Localization Nucleus in Breast Carcinoma, Prostate. Cytoplasm in most malignancies.
Research Area Gene Regulation antibody
Calculated MW 49 kDa

Images (9) Click the Picture to Zoom In

  • ARG53038 anti-FOXA1 / HNF3A antibody [SP88] FACS image

    Flow cytometric: HepG2 cells stained with ARG53038 anti-FOXA1 / HNF3A antibody [SP88] (green) compare to negative control of rabbit IgG (blue).

  • ARG53038 anti-FOXA1 / HNF3A antibody [SP88] IHC-P image

    Immunohistochemistry: Human Bladder Transitional Cell Carcinoma stained with ARG53038 anti-FOXA1 / HNF3A antibody [SP88].

  • ARG53038 anti-FOXA1 / HNF3A antibody [SP88] IHC-P image

    Immunohistochemistry: Human Bladder stained with ARG53038 anti-FOXA1 / HNF3A antibody [SP88].

  • ARG53038 anti-FOXA1 / HNF3A antibody [SP88] IHC-P image

    Immunohistochemistry: Human Breast Adenocarcinoma stained with ARG53038 anti-FOXA1 / HNF3A antibody [SP88].

  • ARG53038 anti-FOXA1 / HNF3A antibody [SP88] IHC-P image

    Immunohistochemistry: Human Breast stained with ARG53038 anti-FOXA1 / HNF3A antibody [SP88].

  • ARG53038 anti-FOXA1 / HNF3A antibody [SP88] IHC-P image

    Immunohistochemistry: Human Lung stained with ARG53038 anti-FOXA1 / HNF3A antibody [SP88].

  • ARG53038 anti-FOXA1 / HNF3A antibody [SP88] IHC-P image

    Immunohistochemistry: Human Ovarian Adenocarcinoma stained with ARG53038 anti-FOXA1 / HNF3A antibody [SP88].

  • ARG53038 anti-FOXA1 / HNF3A antibody [SP88] IHC-P image

    Immunohistochemistry: Human Prostate stained with ARG53038 anti-FOXA1 / HNF3A antibody [SP88].

  • ARG53038 anti-FOXA1 / HNF3A antibody [SP88] IHC-P image

    Immunohistochemistry: Human Prostate stained with ARG53038 anti-FOXA1 / HNF3A antibody [SP88].