ARG63616
anti-FLOT2 / Flotillin 2 antibody
anti-FLOT2 / Flotillin 2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes FLOT2 / Flotillin 2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FLOT2 / Flotillin 2 |
| Antigen Species | Human |
| Immunogen | C-SKIPLIKKATGVQV |
| Conjugation | Un-conjugated |
| Alternate Names | M17S1; ESA1; Membrane component chromosome 17 surface marker 1; ECS-1; Epidermal surface antigen; ECS1; ESA; Flotillin-2 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq, Jul 2008] |
| Research Area | Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 47 kDa |
| PTM | ZDHHC5-catalyzed palmitoylation predominantly occurs at Cys-4. ZDHHC5-catalyzed palmitoylation may be required for the formation of higher-order complexes and for neurite outgrowth in cultured neural stem cells. |
Images (3) Click the Picture to Zoom In
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ARG63616 anti-FLOT2 / Flotillin 2 antibody WB image
Western blot: Human Brain (Cerebellum) lysate (35 µg protein in RIPA buffer) stained with ARG63616 anti-FLOT2 / Flotillin 2 antibody at 0.1 µg/ml dilution.
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ARG63616 anti-FLOT2 / Flotillin 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63616 anti-FLOT2 / Flotillin 2 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63616 anti-FLOT2 / Flotillin 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63616 anti-FLOT2 / Flotillin 2 antibody at 3.75 µg/ml dilution followed by AP-staining.
