ARG54328
anti-FLIP antibody
anti-FLIP antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes FLIP |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody recognizes all human, mouse, and rat FLIP splice variants. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FLIP |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to aa 2-18 of human FLIP (accession no. AAC51622). This sequence is identical in all FLIP splice variants. |
| Conjugation | Un-conjugated |
| Alternate Names | FLAME1; CASP8AP1; c-FLIPL; FLIP; c-FLIPR; c-FLIPS; MACH-related inducer of toxicity; Inhibitor of FLICE; c-FLIP; CASH; Cellular FLICE-like inhibitory protein; CASP8 and FADD-like apoptosis regulator; Caspase homolog; FLAME; FADD-like antiapoptotic molecule 1; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; I-FLICE; FLAME-1; MRIT; Casper; Usurpin; CLARP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa and K562 |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15519 Human CASP8 and FADD-like apoptosis regulator Swiss-port # O35732 Mouse CASP8 and FADD-like apoptosis regulator |
|---|---|
| Gene Symbol | CFLAR |
| Gene Full Name | CASP8 and FADD-like apoptosis regulator |
| Background | Caspase-8 (FLICE) and -10 (FLICE2) are two pivotal members of the ICE/CED-3 protease family. FLICE-inhibitory proteins have been identified in viruses and human cells and are designated v-FLIPs and FLIP, respectively. Human FLIP was cloned by several independent laboratories and designated Casper, I-FLICE, FLAME-1, CASH, and CLARP. FLIP contains two death effector domains and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10 and potently inhibits apoptosis induced by all known death receptors. |
| Function | Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 55 kDa |
| PTM | Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. |
Images (3) Click the Picture to Zoom In
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ARG54328 anti-FLIP antibody IHC-P image
Immunohistochemistry: Mouse liver stained with ARG54328 anti-FLIP antibody at 20 μg/ml dilution.
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ARG54328 anti-FLIP antibody WB image
Western Blot: 1: K562 with antibody at 1 μg/ml; 2: K562 stained with ARG54328 anti-FLIP antibody at 2 μg/ml dilution.
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ARG54328 anti-FLIP antibody ICC/IF image
HeLa stained with ARG54328 anti-FLIP antibody at 5 μg/ml dilution.
Specific References
Wentilactone A induces cell apoptosis by targeting AKR1C1 gene via the IGF‑1R/IRS1/PI3K/AKT/Nrf2/FLIP/Caspase‑3 signaling pathway in small cell lung cancer.
WB / Human
