ARG57088
anti-FLIP antibody [8B12]
anti-FLIP antibody [8B12] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [8B12] recognizes FLIP |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 8B12 |
| Isotype | IgG1, kappa |
| Target Name | FLIP |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-480 of Human FLIP. |
| Conjugation | Un-conjugated |
| Alternate Names | FLAME1; CASP8AP1; c-FLIPL; FLIP; c-FLIPR; c-FLIPS; MACH-related inducer of toxicity; Inhibitor of FLICE; c-FLIP; CASH; Cellular FLICE-like inhibitory protein; CASP8 and FADD-like apoptosis regulator; Caspase homolog; FLAME; FADD-like antiapoptotic molecule 1; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; I-FLICE; FLAME-1; MRIT; Casper; Usurpin; CLARP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15519 Human CASP8 and FADD-like apoptosis regulator |
|---|---|
| Gene Symbol | CFLAR |
| Gene Full Name | CASP8 and FADD-like apoptosis regulator |
| Background | The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011] |
| Function | Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. [UniProt] |
| Calculated MW | 55 kDa |
| PTM | Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. |
Images (2) Click the Picture to Zoom In
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ARG57088 anti-FLIP antibody [8B12] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG57088 anti-FLIP antibody [8B12] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57088 anti-FLIP antibody [8B12] WB image
Western blot: 40 µg of 1) Jurkat cell lysate, 2) HeLa cell lysate, 3) MCF7 cell lysate, 4) K562 cell lysate stained with ARG57088 anti-FLIP antibody [8B12] at 1:1000.
