ARG58621
anti-FGF8 antibody
anti-FGF8 antibody for Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes FGF8 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog, Pig |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FGF8 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from the internal region of Human FGF8 (NP_149355.1; NP_006110.1; NP_149354.1; NP_149353.1). (C-HTTEQSLRFEFLNYP) |
| Conjugation | Un-conjugated |
| Alternate Names | Fibroblast growth factor 8; HH6; AIGF; FGF-8; HBGF-8; KAL6; Heparin-binding growth factor 8; Androgen-induced growth factor |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 25 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FGF8 |
| Gene Full Name | fibroblast growth factor 8 (androgen-induced) |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008] |
| Function | Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Plays a role in neurite outgrowth in hippocampal cells. [UniProt] |
| Calculated MW | 27 kDa |
Images (1) Click the Picture to Zoom In
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ARG58621 anti-FGF8 antibody WB image
Western blot: 35 µg of Jurkat lysate (in RIPA buffer) stained with ARG58621 anti-FGF8 antibody at 2 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
