ARG56817

anti-FGF acidic antibody (Biotin)

anti-FGF acidic antibody (Biotin) for ELISA,Western blot and Human,Mouse

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes FGF acidic
Tested Reactivity Hu, Ms
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FGF acidic
Antigen Species Human
Immunogen E.coli derived Recombinant Human FGF acidic.
(MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D)
Conjugation Biotin
Alternate Names HBGF1; FGF-1; FGF-alpha; GLIO703; ECGFA; ECGFB; ECGF; Endothelial cell growth factor; FGFA; Acidic fibroblast growth factor; Heparin-binding growth factor 1; Fibroblast growth factor 1; HBGF-1; AFGF; aFGF; ECGF-beta

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56708 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14164 Mouse FGF1

GeneID: 2246 Human FGF1

Swiss-port # P05230 Human Fibroblast growth factor 1

Swiss-port # P61148 Mouse Fibroblast growth factor 1

Gene Symbol FGF1
Gene Full Name fibroblast growth factor 1 (acidic)
Background The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt]
Calculated MW 17 kDa
PTM In the nucleus, phosphorylated by PKC/PRKCD.

Images (4) Click the Picture to Zoom In

  • ARG56817 anti-FGF acidic antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human FGF-acidic stained with ARG56817 anti-FGF acidic antibody (Biotin), under reducing conditions.

  • ARG56817 anti-FGF acidic antibody (Biotin) standard curve image

    Direct ELISA: ARG56817 anti-FGF acidic antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.

  • ARG56817 anti-FGF acidic antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human FGF-acidic stained with ARG56817 anti-FGF acidic antibody (Biotin), under non-reducing conditions.

  • ARG56817 anti-FGF acidic antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56817 anti-FGF acidic antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56708 anti-FGF acidic antibody as a capture antibody. Results of a typical standard run with optical density.