ARG56708
anti-FGF acidic antibody
anti-FGF acidic antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes FGF acidic |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ELISA, IHC-P, Neut, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FGF acidic |
| Antigen Species | Human |
| Immunogen | E.coli derived Recombinant Human FGF acidic. (MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D) |
| Conjugation | Un-conjugated |
| Alternate Names | HBGF1; FGF-1; FGF-alpha; GLIO703; ECGFA; ECGFB; ECGF; Endothelial cell growth factor; FGFA; Acidic fibroblast growth factor; Heparin-binding growth factor 1; Fibroblast growth factor 1; HBGF-1; AFGF; aFGF; ECGF-beta |
Application Instructions
| Application Suggestion |
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| Application Note | Please note the application for Neutralizing is lot dependent, if scientist would like to use this antibody for Neutralizing, please contact with arigo. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FGF1 |
| Gene Full Name | fibroblast growth factor 1 (acidic) |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009] |
| Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt] |
| Calculated MW | 17 kDa |
| PTM | In the nucleus, phosphorylated by PKC/PRKCD. |
Images (4) Click the Picture to Zoom In
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ARG56708 anti-FGF acidic antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal kidney. The recommended ARG56708 anti-FGF acidic antibody concentration is 0.050 µg/mL-0.125 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56708 anti-FGF acidic antibody WB image
Western blot: 250 - 0.24 ng of Human FGF-acidic stained with ARG56708 anti-FGF acidic antibody, under reducing conditions.
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ARG56708 anti-FGF acidic antibody WB image
Western blot: 250 - 0.24 ng of Human FGF-acidic stained with ARG56708 anti-FGF acidic antibody, under non-reducing conditions.
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ARG56708 anti-FGF acidic antibody standard curve image
Sandwich ELISA: ARG56708 anti-FGF acidic antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56817 anti-FGF acidic antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
