ARG59309

anti-FBXL11 antibody

anti-FBXL11 antibody for Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes FBXL11
Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FBXL11
Antigen Species Human
Immunogen Synthetic peptide corresponding to a sequence of Human FBXL11. (KRTFDLEEKLHTNKYNANFVTFMEGKDFNVEYIQR).
Conjugation Un-conjugated
Alternate Names CXXC-type zinc finger protein 8; CXXC8; EC 1.14.11.27; LILINA; FBL7; Lysine-specific demethylase 2A; [Histone-H3]-lysine-36 demethylase 1A; FBXL11; JmjC domain-containing histone demethylation protein 1A; JHDM1A; F-box/LRR-repeat protein 11; F-box protein Lilina; F-box protein FBL7; F-box and leucine-rich repeat protein 11; FBL11

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 225876 Mouse KDM2A

GeneID: 22992 Human KDM2A

Swiss-port # P59997 Mouse Lysine-specific demethylase 2A

Swiss-port # Q9Y2K7 Human Lysine-specific demethylase 2A

Gene Symbol KDM2A
Gene Full Name lysine (K)-specific demethylase 2A
Background This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Function Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. [UniProt]
Cellular Localization Nucleus, nucleoplasm. Note=Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2. [UniProt]
Calculated MW 133 kDa

Images (1) Click the Picture to Zoom In

  • ARG59309 anti-FBXL11 antibody WB image

    Western blot: 50 µg of samples under reducing conditions. Mouse liver and A431 cell lysate stained with ARG59309 anti-FBXL11 antibody at 0.5 µg/ml, overnight at 4°C.