ARG42006
anti-FBLN5 / Fibulin 5 antibody
anti-FBLN5 / Fibulin 5 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes FBLN5 / Fibulin 5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FBLN5 / Fibulin 5 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 199-448 of Human FBLN5 / Fibulin 5 (NP_006320.2). |
| Conjugation | Un-conjugated |
| Alternate Names | Urine p50 protein; FIBL-5; DANCE; Dance; ARMD3; EVEC; Developmental arteries and neural crest EGF-like protein; ARCL1A; Fibulin-5; UP50; ADCL2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | A549 | ||||||
| Observed Size | ~ 70 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FBLN5 |
| Gene Full Name | fibulin 5 |
| Background | The protein encoded by this gene is a secreted, extracellular matrix protein containing an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. It is prominently expressed in developing arteries but less so in adult vessels. However, its expression is reinduced in balloon-injured vessels and atherosclerotic lesions, notably in intimal vascular smooth muscle cells and endothelial cells. Therefore, the protein encoded by this gene may play a role in vascular development and remodeling. Defects in this gene are a cause of autosomal dominant cutis laxa, autosomal recessive cutis laxa type I (CL type I), and age-related macular degeneration type 3 (ARMD3). [provided by RefSeq, Jul 2008] |
| Function | Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN. Stabilizes and organizes elastic fibers in the skin, lung and vasculature (By similarity). Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling. [UniProt] |
| Cellular Localization | Secreted. Secreted, extracellular space, extracellular matrix. Note=co-localizes with ELN in elastic fibers. [UniProt] |
| Calculated MW | 50 kDa |
| PTM | N-glycosylated. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG42006 anti-FBLN5 / Fibulin 5 antibody ICC/IF image
Immunofluorescence: L929 cells stained with ARG42006 anti-FBLN5 / Fibulin 5 antibody at 1:100 dilution.
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ARG42006 anti-FBLN5 / Fibulin 5 antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG42006 anti-FBLN5 / Fibulin 5 antibody at 1:1000 dilution.
