ARG43430
anti-FAM120A antibody
anti-FAM120A antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes FAM120A |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1501CT803.19.85.5 |
| Isotype | IgG1, kappa |
| Target Name | FAM120A |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human FAM120A. |
| Conjugation | Un-conjugated |
| Alternate Names | Constitutive coactivator of PPAR-gamma-like protein 1; Protein FAM120A; C9orf10; Oxidative stress-associated Src activator; DNAPTP5; DNAPTP1; OSSA |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | MCF7 | ||||||
| Observed Size | ~ 135 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NZB2 Human Constitutive coactivator of PPAR-gamma-like protein 1 |
|---|---|
| Gene Symbol | FAM120A |
| Gene Full Name | family with sequence similarity 120A |
| Function | May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses. [UniProt] |
| Cellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Translocates to plasma membrane upon ultraviolet exposure. [UniProt] |
| Calculated MW | 122 kDa |
| PTM | Arg-982 is dimethylated, probably to asymmetric dimethylarginine. Phosphorylated on tyrosine by src family kinases upon ultraviolet exposure. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG43430 anti-FAM120A antibody IHC-P image
Immunohistochemistry: Paraformaldehyde-fixed and paraffin-embedded Human brain tissue. Tissue was blocked with 3% BSA for 0.5 hour at room temperature. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG43430 anti-FAM120A antibody at 1:25 dilution for 1 hour at 37°C.
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ARG43430 anti-FAM120A antibody WB image
Western blot: 20 µg of MCF7 whole cell lysate stained with ARG43430 anti-FAM120A antibody at 1:4000 dilution.
