ARG41838

anti-FADD antibody

anti-FADD antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes FADD
Tested Reactivity Ms
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FADD
Antigen Species Human
Immunogen Synthetic peptide of Human FADD.
Conjugation Un-conjugated
Alternate Names Mediator of receptor induced toxicity; MORT1; GIG3; FAS-associated death domain protein; Growth-inhibiting gene 3 protein; Protein FADD; FAS-associating death domain-containing protein

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Raw264.7
Observed Size ~ 26 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14082 Mouse FADD

Swiss-port # Q61160 Mouse FAS-associated death domain protein

Gene Symbol FADD
Gene Full Name Fas (TNFRSF6)-associated via death domain
Background The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]
Function Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling. [UniProt]
Calculated MW 23 kDa

Images (1) Click the Picture to Zoom In

  • ARG41838 anti-FADD antibody WB image

    Western blot: Raw264.7 cell lysate stained with ARG41838 anti-FADD antibody.