ARG64242
anti-FACT / SUPT16H antibody
anti-FACT / SUPT16H antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes FACT / SUPT16H |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Cow, Pig |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FACT / SUPT16H |
| Antigen Species | Human |
| Immunogen | C-DLTNKEGKKPEEK |
| Conjugation | Un-conjugated |
| Alternate Names | Chromatin-specific transcription elongation factor 140 kDa subunit; FACTP140; FACT complex subunit SPT16; SPT16; FACTp140; CDC68; FACT 140 kDa subunit; Facilitates chromatin transcription complex subunit SPT16; SPT16/CDC68; hSPT16 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009] |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 120 kDa |
| PTM | ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin. |
Images (1) Click the Picture to Zoom In
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ARG64242 anti-FACT / SUPT16H antibody WB image
Western Blot: HeLa cell lysate (35 µg protein in RIPA buffer) stained with ARG64242 anti-FACT / SUPT16H antibody at 0.1 µg/ml dilution.
