ARG64176
anti-FABP2 / Intestinal FABP antibody
anti-FABP2 / Intestinal FABP antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes FABP2 / Intestinal FABP |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FABP2 / Intestinal FABP |
| Antigen Species | Human |
| Immunogen | C-EGVEAKRIFKKD |
| Conjugation | Un-conjugated |
| Alternate Names | Intestinal-type fatty acid-binding protein; I-FABP; Fatty acid-binding protein, intestinal; Fatty acid-binding protein 2; FABPI |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P12104 Human Fatty acid-binding protein, intestinal |
|---|---|
| Background | The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 15 kDa |
Images (3) Click the Picture to Zoom In
-
ARG64176 anti-FABP2 / Intestinal FABP antibody WB image
Western blot: Human Duodenum lysate (35 µg protein in RIPA buffer) stained with ARG64176 anti-FABP2 / Intestinal FABP antibody at 0.001 µg/ml dilution.
-
ARG64176 anti-FABP2 / Intestinal FABP antibody WB image
Western blot: 1). Mock transfection; 2) FABP2 (RC210206) expressing plasmid transfected HEK293 cell lysate standed with ARG64176 anti-FABP2 / Intestinal FABP antibody.
-
ARG64176 anti-FABP2 / Intestinal FABP antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Small Intestine. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64176 anti-FABP2 / Intestinal FABP antibody at 2.5 µg/ml dilution followed by AP-staining.
