ARG63968
anti-Ezrin antibody
anti-Ezrin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Ezrin |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Ezrin |
| Antigen Species | Human |
| Immunogen | C-QDYEEKTKKAER |
| Conjugation | Un-conjugated |
| Alternate Names | Ezrin; p81; HEL-S-105; VIL2; CVL; Cytovillin; CVIL; Villin-2 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008] |
| Highlight | Related Antibody Duos and Panels: ARG30231 Phospho Ezrin Antibody Duo (Total, pT567) Related products: Ezrin antibodies; Ezrin Duos / Panels; Anti-Goat IgG secondary antibodies; |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 69 kDa |
| PTM | Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity). S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
Images (3) Click the Picture to Zoom In
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ARG63968 anti-Ezrin antibody WB image
Western blot: Human Placenta lysate (35 µg protein in RIPA buffer) stained with ARG63968 anti-Ezrin antibody at 1 µg/ml dilution.
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ARG63968 anti-Ezrin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63968 anti-Ezrin antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63968 anti-Ezrin antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Small Intestine. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63968 anti-Ezrin antibody at 3.8 µg/ml dilution followed by AP-staining.
