ARG57094
anti-Epoxide Hydrolase antibody [2E5]
anti-Epoxide Hydrolase antibody [2E5] for ICC/IF,Western blot and Human,Mouse
Overview
| Product Description | Mouse Monoclonal antibody [2E5] recognizes Epoxide Hydrolase |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2E5 |
| Isotype | IgG2a, kappa |
| Target Name | Epoxide Hydrolase |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 21-455 of Human Epoxide Hydrolase |
| Conjugation | Un-conjugated |
| Alternate Names | EPHX; Epoxide hydratase; Epoxide hydrolase 1; EPOX; EC 3.3.2.9; MEH; Microsomal epoxide hydrolase; HYL1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EPHX1 |
| Gene Full Name | epoxide hydrolase 1, microsomal (xenobiotic) |
| Background | Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2008] |
| Function | Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. [UniProt] |
| Calculated MW | 53 kDa |
Images (3) Click the Picture to Zoom In
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ARG57094 anti-Epoxide Hydrolase antibody [2E5] ICC/IF image
Immunofluorescence: Hep3B cells line stained with ARG57094 anti-Epoxide Hydrolase antibody [2E5] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57094 anti-Epoxide Hydrolase antibody [2E5] WB image
Western blot: 30 µg of Mouse liver lysate stained with ARG57094 anti-Epoxide Hydrolase antibody [2E5] at 1:1000.
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ARG57094 anti-Epoxide Hydrolase antibody [2E5] WB image
Western blot: 10 µg of 1) 293T, and 2) EPHX1 transfected 293T cell lysates stained with ARG57094 anti-Epoxide Hydrolase antibody [2E5] at 1:500.
