ARG66335
anti-EpCAM antibody [SQab1883]
anti-EpCAM antibody [SQab1883] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1883] recognizes EpCAM |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1883 |
| Isotype | IgG |
| Target Name | EpCAM |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 1-100 of Human EPCAM. |
| Conjugation | Un-conjugated |
| Alternate Names | MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EPCAM |
| Gene Full Name | epithelial cell adhesion molecule |
| Background | EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008] |
| Function | EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt] |
| Highlight | Related products: EPCAM antibodies; EPCAM ELISA Kits; EPCAM Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| Research Area | Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody |
| Calculated MW | 35 kDa |
| PTM | Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG66335 anti-EpCAM antibody [SQab1883] FACS image
Flow Cytometry: HCT-116 cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66335 anti-EpCAM antibody [SQab1883] (red) at 1:800 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66335 anti-EpCAM antibody [SQab1883] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human uterus tissue stained with ARG66335 anti-EpCAM antibody [SQab1883] at 1:3,200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
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ARG66335 anti-EpCAM antibody [SQab1883] WB image
Western blot: 10 µg of HT-29 and T47D cell lysates stained with ARG66335 anti-EpCAM antibody [SQab1883] at 1:2000 dilution.
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ARG66335 anti-EpCAM antibody [SQab1883] IP image
Immunoprecipitation: 0.4 mg of HT-29 whole cell lysate immunoprecipitated (1:50) and stained with ARG66335 anti-EpCAM antibody [SQab1883]. 1) ARG66335 IP in HT-29 whole cell lysate, 2) PBS instead of ARG66335 in HT-29 whole cell lysate, and 3) HT-29 whole cell lysate, 10 µg (input).
