ARG56049

anti-EpCAM antibody [MOC-31]

anti-EpCAM antibody [MOC-31] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody

Overview

Product Description Mouse Monoclonal antibody [MOC-31] recognizes EpCAM
Tested Reactivity Hu
Species Does Not React With Rat
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone MOC-31
Isotype IgG1, kappa
Target Name EpCAM
Antigen Species Human
Immunogen Neuraminidase treated GLS-1 Human small cell lung carcinoma cells.
Conjugation Un-conjugated
Alternate Names MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 - 5 µg/ml
WB1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4072 Human EPCAM

Swiss-port # P16422 Human Epithelial cell adhesion molecule

Gene Symbol EPCAM
Gene Full Name epithelial cell adhesion molecule
Background EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Function EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]
Cellular Localization Cell surface
Research Area Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
Calculated MW 35 kDa
PTM Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.

Images (3) Click the Picture to Zoom In

  • ARG56049 anti-EpCAM antibody [MOC-31] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human endometrial carcinoma stained with ARG56049 anti-EpCAM antibody [MOC-31].

  • ARG56049 anti-EpCAM antibody [MOC-31] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human ovarian carcinoma stained with ARG56049 anti-EpCAM antibody [MOC-31].

  • ARG56049 anti-EpCAM antibody [MOC-31] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human colon carcinoma stained with ARG56049 anti-EpCAM antibody [MOC-31].