ARG56049

anti-EpCAM antibody [MOC-31]

Datasheet

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50 μg

379.00

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Datasheet

Overview

Product Description	Mouse Monoclonal antibody [MOC-31] recognizes EpCAM
Tested Reactivity	Hu
Species Does Not React With	Rat
Tested Application	IHC-P, WB
Host	Mouse
Clonality	Monoclonal
Clone	MOC-31
Isotype	IgG1, kappa
Target Name	EpCAM
Antigen Species	Human
Immunogen	Neuraminidase treated GLS-1 Human small cell lung carcinoma cells.
Conjugation	Un-conjugated
Alternate Names	MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326

Application Instructions

Application Suggestion

Tested Application	Dilution
IHC-P	2 - 5 µg/ml
WB	1 - 2 µg/ml

Application Note

IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purification with Protein G.
Buffer	PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative	0.05% Sodium azide
Stabilizer	0.1 mg/ml BSA
Concentration	0.2 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 4072 Human EPCAM Swiss-port # P16422 Human Epithelial cell adhesion molecule
Gene Symbol	EPCAM
Gene Full Name	epithelial cell adhesion molecule
Background	EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Function	EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]
Cellular Localization	Cell surface
Research Area	Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
Calculated MW	35 kDa
PTM	Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.

Images (3) Click the Picture to Zoom In

ARG56049 anti-EpCAM antibody [MOC-31] IHC-P image

Immunohistochemistry: Formalin-fixed, paraffin-embedded Human endometrial carcinoma stained with ARG56049 anti-EpCAM antibody [MOC-31].
ARG56049 anti-EpCAM antibody [MOC-31] IHC-P image

Immunohistochemistry: Formalin-fixed, paraffin-embedded Human ovarian carcinoma stained with ARG56049 anti-EpCAM antibody [MOC-31].
ARG56049 anti-EpCAM antibody [MOC-31] IHC-P image

Immunohistochemistry: Formalin-fixed, paraffin-embedded Human colon carcinoma stained with ARG56049 anti-EpCAM antibody [MOC-31].