ARG43760

anti-EpCAM antibody

anti-EpCAM antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Mouse,Rat

Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes EpCAM
Tested Reactivity Ms, Rat
Tested Application ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name EpCAM
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to a.a. E147-N189 in extracellular domain of Mouse EpCAM.
Conjugation Un-conjugated
Alternate Names MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 40 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.005% Sodium azide and 4% Trehalose.
Preservative 0.005% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17075 Mouse EPCAM

GeneID: 171577 Rat EPCAM

Swiss-port # O55159 Rat Epithelial cell adhesion molecule

Swiss-port # Q99JW5 Mouse Epithelial cell adhesion molecule

Gene Symbol EPCAM
Gene Full Name epithelial cell adhesion molecule
Background EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Function EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]
Cellular Localization Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction. [UniProt]
Research Area Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
Calculated MW 35 kDa
PTM Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. [UniProt]