ARG57167
anti-Enolase 1/2 antibody [1G7]
anti-Enolase 1/2 antibody [1G7] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [1G7] recognizes Enolase 1/2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1G7 |
| Isotype | IgG2a, kappa |
| Target Name | Enolase 1/2 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-434 of Human Enolase1/2 |
| Conjugation | Un-conjugated |
| Alternate Names | MPB1; Plasminogen-binding protein; Alpha-enolase; MBP-1; NNE; PPH; Enolase 1; ENO1L1; Phosphopyruvate hydratase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Non-neural enolase; MPB-1; EC 4.2.1.11 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ENO1 |
| Gene Full Name | enolase 1, (alpha) |
| Background | This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011] |
| Function | Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. [UniProt] |
| Calculated MW | 47 kDa |
| PTM | ISGylated. |
Images (4) Click the Picture to Zoom In
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ARG57167 anti-Enolase 1/2 antibody [1G7] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57167 anti-Enolase 1/2 antibody [1G7] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57167 anti-Enolase 1/2 antibody [1G7] FACS image
Flow Cytometry: LNCap cell line stained with ARG57167 anti-Enolase 1/2 antibody [1G7] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57167 anti-Enolase 1/2 antibody [1G7] WB image
Western blot: 50 ng of 1) ENO1 (Alpha-enolase), 2) ENO2 (Gamma-enolase), and 3) ENO3 (Beta-enolase) recombinant proteins stained with ARG57167 anti-Enolase 1/2 antibody [1G7] at 1:1000.
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ARG57167 anti-Enolase 1/2 antibody [1G7] WB image
Western blot: 40 µg of 1) PC3, 2) MCF7, 3) 293T, and 4) HeLa cell lysates stained with ARG57167 anti-Enolase 1/2 antibody [1G7] at 1:1000.
