ARG58589
anti-Endothelin B Receptor antibody
anti-Endothelin B Receptor antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
Product Description | Rabbit Polyclonal antibody recognizes Endothelin B Receptor |
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Tested Reactivity | Hu, Ms, Rat |
Tested Application | FACS, ICC/IF, IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | Endothelin B Receptor |
Antigen Species | Human |
Immunogen | Synthetic peptide corresponding to aa. 406-439 of Human Endothelin B Receptor. (QSFEEKQSLEEKQSCLKFKANDHGYDNFRSSNKY) |
Conjugation | Un-conjugated |
Alternate Names | HSCR; WS4A; HSCR2; ETB1; Endothelin receptor non-selective type; ETRB; ETB; ET-B; ET-BR; Endothelin B receptor; ABCDS; ETBR |
Application Instructions
Application Suggestion |
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Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Affinity purification with immunogen. |
Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
Preservative | 0.05% Sodium azide |
Stabilizer | 5% BSA |
Concentration | 0.5 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | EDNRB |
Gene Full Name | endothelin receptor type B |
Background | The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
Function | Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. [UniProt] |
Cellular Localization | Cell membrane; Multi-pass membrane protein. [UniProt] |
Calculated MW | 50 kDa |
PTM | Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG58589 anti-Endothelin B Receptor antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG58589 anti-Endothelin B Receptor antibody (red) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG58589 anti-Endothelin B Receptor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse Testis Tissue stained with ARG58589 anti-Endothelin B Receptor antibody.
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ARG58589 anti-Endothelin B Receptor antibody WB image
Western blot: 40 µg of HeLa whole cell lysate stained with ARG58589 anti-Endothelin B Receptor antibody at 0.5 µg/ml dilution.
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ARG58589 anti-Endothelin B Receptor antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG58589 anti-Endothelin B Receptor antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58589 anti-Endothelin B Receptor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat Testis Tissue stained with ARG58589 anti-Endothelin B Receptor antibody.
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ARG58589 anti-Endothelin B Receptor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human Placenta Tissue stained with ARG58589 anti-Endothelin B Receptor antibody.