ARG54437
anti-EndoG antibody
anti-EndoG antibody for ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes EndoG |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC, WB |
| Specificity | This antibody recognizes human, mouse, and rat EndoG (Endonuclease G) an enzyme that is activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondria-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EndoG |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to aa 55-70 of human EndoG (accession no. NP_004426). |
| Conjugation | Un-conjugated |
| Alternate Names | EC 3.1.30.-; Endo G; Endonuclease G, mitochondrial |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | 3T3, HepG2 and Human pancreas |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ENDOG |
| Gene Full Name | endonuclease G |
| Background | The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq, Jul 2008] |
| Function | Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (By similarity). [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody |
| Calculated MW | 33 kDa |
Images (2) Click the Picture to Zoom In
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ARG54437 anti-EndoG antibody WB image
Western blot: 30 µg of U87 and HepG2 cell lysates stained with ARG54437 anti-EndoG antibody at 1:500 dilution.
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ARG54437 anti-EndoG antibody IHC image
Immunohistochemistry: human pancreas stained with ARG54437 anti-EndoG antibody at 15 μg/ml dilution.
Customer's Feedback
Good
Review for anti-EndoG antibody
Application：WB
Sample：U87 and HepG2
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
