ARG59025
anti-Elafin antibody [C7]
anti-Elafin antibody [C7] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Mouse Monoclonal antibody [C7] recognizes Elafin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | C7 |
| Isotype | IgG1 |
| Target Name | Elafin |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to A61-Q117 of Human Elafin. |
| Conjugation | Un-conjugated |
| Alternate Names | Protease inhibitor WAP3; cementoin; WFDC14; Skin-derived antileukoproteinase; WAP four-disulfide core domain protein 14; SKALP; Elafin; Elastase-specific inhibitor; WAP3; Peptidase inhibitor 3; ESI; PI-3 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PI3 |
| Gene Full Name | peptidase inhibitor 3, skin-derived |
| Background | This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2014] |
| Function | Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 12 kDa |
Images (1) Click the Picture to Zoom In
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ARG59025 anti-Elafin antibody [C7] IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59025 anti-Elafin antibody [C7] at 2 µg/ml dilution, overnight at 4°C.
