ARG53992
anti-Eg 5 antibody
anti-Eg 5 antibody for ICC/IF,Immunoprecipitation,Western blot and Human
Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes Eg 5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | Eg 5 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human Eg5 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | TRIP-5; Kinesin-related motor protein Eg5; KNSL1; TR-interacting protein 5; HKSP; Kinesin-like spindle protein HKSP; EG5; MCLMR; Kinesin-like protein 1; Kinesin-like protein KIF11; TRIP5; Thyroid receptor-interacting protein 5 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 130 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | Lot dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KIF11 |
| Gene Full Name | kinesin family member 11 |
| Background | Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays. |
| Function | Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays. [UniProt] |
| Cellular Localization | Cytoplasm |
| Research Area | Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 119 kDa |
| PTM | Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function. |
Images (3) Click the Picture to Zoom In
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ARG53992 anti-Eg 5 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG53992 anti-Eg 5 antibody at 1:200 dilution.
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ARG53992 anti-Eg 5 antibody WB image
Western blot: MCF7 cell lysate stained with ARG53992 anti-Eg 5 antibody at 1:1000 dilution.
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ARG53992 anti-Eg 5 antibody IP image
Immunoprecipitation: Jurkat cell lysates were immunoprecipitated and stained with ARG53992 anti-Eg 5 antibody.
