ARG55271
anti-EZH1 antibody
anti-EZH1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes EZH1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | IHC-P, WB |
| Specificity | Multiple isoforms of EZH1 are known to exist. EZH1 antibody is predicted to not cross-react with EZH2 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EZH1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (18 aa) within aa. 180-230 of Human EZH1. |
| Conjugation | Un-conjugated |
| Alternate Names | Enhancer of zeste homolog 1; ENX-2; EC 2.1.1.43; KMT6B; Histone-lysine N-methyltransferase EZH1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse Lung Tissue Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P70351 Mouse Histone-lysine N-methyltransferase EZH1 Swiss-port # Q92800 Human Histone-lysine N-methyltransferase EZH1 |
|---|---|
| Gene Symbol | EZH1 |
| Gene Full Name | enhancer of zeste 1 polycomb repressive complex 2 subunit |
| Background | EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009] |
| Function | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 85 kDa |
Images (3) Click the Picture to Zoom In
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ARG55271 anti-EZH1 antibody WB image
Western blot: Mouse lung tissue lysate stained with ARG55271 anti-EZH1 antibody at (A) 1 and (B) 2 µg/ml dilution.
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ARG55271 anti-EZH1 antibody IHC image
Immunohistochemistry: Human lung tissue stained with ARG55271 anti-EZH1 antibody at 20 µg/ml dilution.
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ARG55271 anti-EZH1 antibody IHC-P image
Immunohistochemistry: Human lung tissue stained with ARG55271 anti-EZH1 antibody at 5 µg/ml dilution.
