ARG65147
anti-EVI1 / AML1 antibody
anti-EVI1 / AML1 antibody for Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes EVI1 / AML1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize all reported isoforms (NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1). Reported variants represent identical protein: NP_001098548.2, NP_005232.2 |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EVI1 / AML1 |
| Antigen Species | Human |
| Immunogen | C-DKESLHSTSH |
| Conjugation | Un-conjugated |
| Alternate Names | MDS1 and EVI1 complex locus protein EVI1; AML1-EVI-1; Ecotropic virus integration site 1 protein homolog; EVI1; MDS1-EVI1; PRDM3; MDS1; EVI-1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q03112 Human MDS1 and EVI1 complex locus protein EVI1 |
|---|---|
| Background | The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 138 kDa |
| PTM | Phosphorylated. May be acetylated by CREBBP and KAT2B. |
Images (1) Click the Picture to Zoom In
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ARG65147 anti-EVI1 / AML1 antibody WB image
Western Blot: A549 lysate (35 µg protein in RIPA buffer) stained with ARG65147 anti-EVI1 antibody at 2 µg/ml dilution.
