ARG43520
anti-ERCC2 / XPD antibody
anti-ERCC2 / XPD antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ERCC2 / XPD |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ERCC2 / XPD |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human ERCC2 / XPD. |
| Conjugation | Un-conjugated |
| Alternate Names | ACTN2; Actinin Alpha 2; F-Actin Cross-Linking Protein; Alpha-Actinin-2; Alpha-Actinin Skeletal Muscle Isoform 2; Alpha-Actinin Skeletal Muscle; CMD1AA; MYOCOZ; CMH23; CMYO8; CMYP8; MPD6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 87 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O08811 Mouse TFIIH basal transcription factor complex helicase XPD subunit Swiss-port # P18074 Human TFIIH basal transcription factor complex helicase XPD subunit |
|---|---|
| Gene Symbol | ERCC2 |
| Gene Full Name | excision repair cross-complementation group 2 |
| Background | The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| Function | ATP-dependent 5'-3' DNA helicase, component of the core-TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers. [UniProt] |
| Cellular Localization | Cytoplasm; Cytoskeleton; Nucleus. [UniProt] |
| Calculated MW | 87 kDa |
| PTM | Ubl conjugation. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG43520 anti-ERCC2 / XPD antibody IHC-P image
Immunohistochemistry: Human bladder epithelial carcinom stained with ARG43520 anti-ERCC2 / XPD antibody at 2 μg/ml dilution.
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ARG43520 anti-ERCC2 / XPD antibody WB image
Western blot: K562 and SiHa stained with ARG43520 anti-ERCC2 / XPD antibody at 0.5 μg/ml dilution.
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ARG43520 anti-ERCC2 / XPD antibody FACS image
Flow Cytometry: THP-1 stained with ARG43520 anti-ERCC2 / XPD antibody at 1 µg/10^6 cells dilution.
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ARG43520 anti-ERCC2 / XPD antibody IHC-P image
Immunohistochemistry: Rat testis stained with ARG43520 anti-ERCC2 / XPD antibody at 2 μg/ml dilution.
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ARG43520 anti-ERCC2 / XPD antibody IHC-P image
Immunohistochemistry: Mouse testis stained with ARG43520 anti-ERCC2 / XPD antibody at 2 μg/ml dilution.
