ARG62480
anti-ERCC1 antibody [3H11]
anti-ERCC1 antibody [3H11] for Immunoprecipitation,Western blot and Human
Cancer antibody; Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [3H11] recognizes ERCC1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3H11 |
| Isotype | IgG2a |
| Target Name | ERCC1 |
| Antigen Species | Human |
| Immunogen | His-tagged recombinant human ERCC1 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | DNA excision repair protein ERCC-1; RAD10; COFS4; UV20 |
Application Instructions
| Application Note | WB: 1/375 - 1/750 IP: 2 µg/mg of lysate * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purified |
| Buffer | 1X PBS buffer with <0.1% sodium azide. |
| Preservative | <0.1% sodium azide. |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P07992 Human DNA excision repair protein ERCC-1 |
|---|---|
| Gene Symbol | ERCC1 |
| Gene Full Name | excision repair cross-complementation group 1 |
| Background | The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009] |
| Function | Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. [UniProt] |
| Cellular Localization | Nucleus |
| Research Area | Cancer antibody; Gene Regulation antibody |
| Calculated MW | 33 kDa |
Clone References
Subcellular distribution of a fluorescence-labeled combi-molecule designed to block epidermal growth factor receptor tyrosine kinase and damage DNA with a green fluorescent species.
WB / Human
The ERCC1/XPF endonuclease is required for completion of homologous recombination at DNA replication forks stalled by inter-strand cross-links.
WB / Human
Development of an unsupervised pixel-based clustering algorithm for compartmentalization of immunohistochemical expression using Automated QUantitative Analysis.
IHC / Human
ERCC1 expression and RAD51B activity correlate with cell cycle response to platinum drug treatment not DNA repair.
WB / Human
Tumors established with cell lines selected for oxaliplatin resistance respond to oxaliplatin if combined with cetuximab.
WB / Human
