ARG58775

anti-ERBB2 / HER2 antibody

anti-ERBB2 / HER2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody; Circulating Tumor Cells BioMarker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ERBB2 / HER2
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ERBB2 / HER2
Antigen Species Human
Immunogen Synthetic peptide of Human HER2 / ErbB2.
Conjugation Un-conjugated
Alternate Names p185erbB2; Proto-oncogene c-ErbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; NGL; EC 2.7.10.1; CD340; CD antigen CD340; TKR1; HER-2; Tyrosine kinase-type cell surface receptor HER2; HER2; NEU; HER-2/neu; MLN 19; Receptor tyrosine-protein kinase erbB-2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse uterus
Observed Size 185 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13866 Mouse ERBB2

GeneID: 2064 Human ERBB2

Swiss-port # P04626 Human Receptor tyrosine-protein kinase erbB-2

Swiss-port # P70424 Mouse Receptor tyrosine-protein kinase erbB-2

Gene Symbol ERBB2
Gene Full Name erb-b2 receptor tyrosine kinase 2
Background This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
Function Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. [UniProt]
Cellular Localization Cell membrane, Single-pass type I membrane protein, Cytoplasm, perinuclear region, Nucleus, Nucleus. [UniProt]
Research Area Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody; Circulating Tumor Cells BioMarker antibody
Calculated MW 138 kDa
PTM Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG58775 anti-ERBB2 / HER2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung cancer stained with ARG58775 anti-ERBB2 / HER2 antibody at 1:100 dilution.

  • ARG58775 anti-ERBB2 / HER2 antibody WB image

    Western blot: 25 µg of Mouse uterus lysate stained with ARG58775 anti-ERBB2 / HER2 antibody at 1:500 dilution.