ARG54982
anti-ERBB2 / HER2 antibody
anti-ERBB2 / HER2 antibody for Western blot and Human,Mouse,Rat
Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody; Circulating Tumor Cells BioMarker antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ERBB2 / HER2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ERBB2 / HER2 |
| Antigen Species | Mouse |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 1236-1268 of Mouse ERBB2. |
| Conjugation | Un-conjugated |
| Alternate Names | p185erbB2; Proto-oncogene c-ErbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; NGL; EC 2.7.10.1; CD340; CD antigen CD340; TKR1; HER-2; Tyrosine kinase-type cell surface receptor HER2; HER2; NEU; HER-2/neu; MLN 19; Receptor tyrosine-protein kinase erbB-2 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Rat lung |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P04626 Human Receptor tyrosine-protein kinase erbB-2 Swiss-port # P70424 Mouse Receptor tyrosine-protein kinase erbB-2 |
|---|---|
| Gene Symbol | Erbb2 |
| Gene Full Name | v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) |
| Background | Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). |
| Research Area | Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody; Circulating Tumor Cells BioMarker antibody |
| Calculated MW | 138 kDa |
| PTM | Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). |
Images (1) Click the Picture to Zoom In
-
ARG54982 anti-ERBB2 / HER2 antibody WB image
Western blot: 20 µg of Rat lung lysate stained with ARG54982 anti-ERBB2 / HER2 antibody at 1:2000 dilution.
