Overview

Product Description	Rabbit Polyclonal antibody recognizes ERAB / HADH2
Tested Reactivity	Hu
Predict Reactivity	Ms, Rat
Tested Application	FACS, ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	ERAB / HADH2
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human ERAB / HADH2.
Conjugation	Un-conjugated
Alternate Names	MRPP2; ERAB; ABAD; Short chain dehydrogenase/reductase family 5C member 1; HCD2; SDR5C1; 3-hydroxyacyl-CoA dehydrogenase type-2; 17-beta-hydroxysteroid dehydrogenase 10; SCHAD; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; EC 1.1.1.178; Short-chain type dehydrogenase/reductase XH98G2; Endoplasmic reticulum-associated amyloid beta-peptide-binding protein; 17-beta-HSD 10; CAMR; Mitochondrial RNase P protein 2; EC 1.1.1.35; Type II HADH; 3-hydroxyacyl-CoA dehydrogenase type II; EC 1.1.1.51; 17b-HSD10; MRX31; DUPXp11.22; HADH2; MRX17; MRXS10; Mitochondrial ribonuclease P protein 2; MHBD

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:50 ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:1000 - 1:5000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HeLa
Observed Size	~ 25 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 3028 Human HSD17B10 Swiss-port # Q99714 Human 3-hydroxyacyl-CoA dehydrogenase type-2
Gene Symbol	HSD17B10
Gene Full Name	hydroxysteroid (17-beta) dehydrogenase 10
Background	This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Aug 2014]
Function	Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. Catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. Catalyzes the third step in the beta-oxidation of fatty acids. Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids. Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD). [UniProt]
Cellular Localization	Mitochondrion. [UniProt]
Calculated MW	27 kDa

Images (1) Click the Picture to Zoom In