ARG22201
anti-ENaC beta antibody
anti-ENaC beta antibody for ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat,Hamster,Xenopus laevis
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ENaC beta |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Hm, Xenopus laevis |
| Tested Application | ICC/IF, IHC, IP, WB |
| Specificity | Detects ~87kDa. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ENaC beta |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide around aa. 617-638 (C-terminus) of Rat ENaC Beta |
| Conjugation | Un-conjugated |
| Alternate Names | Beta-ENaC; Nonvoltage-gated sodium channel 1 subunit beta; Epithelial Na; ENaCb; Amiloride-sensitive sodium channel subunit beta; ENaCB; Beta-NaCH; SCNEB; BESC1; ENaCbeta |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Scnn1b |
| Gene Full Name | sodium channel, non-voltage-gated 1, beta subunit |
| Background | Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the beta subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), and Liddle syndrome. [provided by RefSeq, Apr 2009] |
| Function | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. [UniProt] |
| Cellular Localization | Apical cell membrane |
| Calculated MW | 73 kDa |
| PTM | Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation. N-glycosylated. N-glycosylation is required for interaction with BPIFA1. |
Images (4) Click the Picture to Zoom In
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ARG22201 anti-ENaC beta antibody IHC image
Immunohistochemistry: Rat kidney tissue stained with ARG22201 anti-ENaC beta antibody.
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ARG22201 anti-ENaC beta antibody WB image
Western blot: Rat kidney tissue lysates stained with ARG22201 anti-ENaC beta antibody at 1:1000 dilution.
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ARG22201 anti-ENaC beta antibody WB image
Western blot: 1) Mouse mpkCCD cell lysate, and 2) FRT expressing tagged beta-mENaC stained with ARG22201 anti-ENaC beta antibody.
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ARG22201 anti-ENaC beta antibody WB image
Western blot: Mouse kidney cortex stained with ARG22201 anti-ENaC beta antibody at 1:1000 dilution. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-8).
