ARG42130

anti-ENC1 antibody

anti-ENC1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat

Overview

Product Description Chicken Polyclonal antibody recognizes ENC1
Tested Reactivity Ms, Rat
Tested Application IHC-P, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name ENC1
Antigen Species Human
Immunogen A 13 amino acid synthetic peptide within aa. 230 - 280 of Human ENC1.
Conjugation Un-conjugated
Alternate Names KLHL37; KLHL35; Kelch-like protein 37; TP53I10; PIG10; NRPB; Nuclear matrix protein NRP/B; CCL28; ENC-1; Ectoderm-neural cortex protein 1; p53-induced gene 10 protein

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 - 20 µg/ml
WB1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 51 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13803 Mouse ENC1

Swiss-port # O35709 Mouse Ectoderm-neural cortex protein 1

Gene Symbol ENC1
Gene Full Name ectodermal-neural cortex 1 (with BTB domain)
Background This gene encodes a member of the kelch-related family of actin-binding proteins. The encoded protein plays a role in the oxidative stress response as a regulator of the transcription factor Nrf2, and expression of this gene may play a role in malignant transformation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
Function Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. Down-regulates transcription factor NF2L2/NRF2 by decreasing the rate of protein synthesis and not via a ubiquitin-mediated proteasomal degradation mechanism. [UniProt]
Cellular Localization Nucleus matrix. Cytoplasm. Cytoplasm, cytoskeleton. [UniProt]
Calculated MW 66 kDa
PTM Ubiquitinated by E3 ubiquitin ligase complex formed by CUL3 and RBX1 and probably targeted for proteasome-independent degradation. Quinone-induced oxidative stress increases its ubiquitination. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG42130 anti-ENC1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG42130 anti-ENC1 antibody at 5 µg/ml dilution.

  • ARG42130 anti-ENC1 antibody WB image

    Western blot: Mouse kidney muscle lysate stained with ARG42130 anti-ENC1 antibody at 1 µg/ml dilution, in the absence (left) and the presence (right) of blocking peptide.

  • ARG42130 anti-ENC1 antibody IHC image

    Immunohistochemistry: Rat kidney tissue stained with ARG42130 anti-ENC1 antibody at 20 µg/ml dilution.