ARG22473
anti-EMR1 F4/80 antibody [Cl:A3-1] (low endotoxin)
anti-EMR1 F4/80 antibody [Cl:A3-1] (low endotoxin) for Flow cytometry,Functional study,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Radioimmunoassay,Western blot and Mouse
Mouse Inflammatory Cell Marker antibody; Macrophage Marker antibody
Overview
| Product Description | Azide free and low endotoxin Rat Monoclonal antibody [Cl:A3-1] recognizes EMR1 F4/80 This antibody recognizes the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, FuncSt, IHC-Fr, IHC-P, IP, RIA, WB |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | Cl:A3-1 |
| Isotype | IgG2b |
| Target Name | EMR1 F4/80 |
| Antigen Species | Mouse |
| Immunogen | Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice. |
| Conjugation | Un-conjugated |
| Alternate Names | EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; EMR1; Adhesion G protein-coupled receptor E1; EGF-like module receptor 1; TM7LN3 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques. FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Purification Note | Low endotoxin |
| Buffer | PBS |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q61549 Mouse Adhesion G protein-coupled receptor E1 |
|---|---|
| Gene Symbol | Adgre1 |
| Gene Full Name | adhesion G protein-coupled receptor E1 |
| Background | EMR1 F4/80 has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| Function | EMR1 F4/80: Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. [UniProt] |
| Highlight | Related products: EMR1 F4/80 antibodies; EMR1 F4/80 Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| Research Area | Mouse Inflammatory Cell Marker antibody; Macrophage Marker antibody |
| Calculated MW | 98 kDa |
