ARG63882
anti-EMI2 antibody
anti-EMI2 antibody for Western blot and Mouse
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes EMI2 |
|---|---|
| Tested Reactivity | Ms |
| Predict Reactivity | Hu, Rat, Cow, Dog, Pig |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EMI2 |
| Antigen Species | Human |
| Immunogen | C-PGSAQSKRNLKRL |
| Conjugation | Un-conjugated |
| Alternate Names | ERP1; FBX43; EMI2; F-box only protein 43; Endogenous meiotic inhibitor 2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008] |
|---|---|
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 78 kDa |
| PTM | Phosphorylated on Ser-76, Thr-234 and Ser-334 in response to calcium, which is a prerequisite for ubiquitination and proteasomal degradation. Ubiquitinated in response to calcium, which promotes proteasomal degradation. |
Images (1) Click the Picture to Zoom In
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ARG63882 anti-EMI2 antibody WB image
Western blot: 35 µg of Mouse ovary lysate (in RIPA buffer) stained with ARG63882 anti-EMI2 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
