ARG63176
anti-ELMO1 antibody
anti-ELMO1 antibody for Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ELMO1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog, Zfsh |
| Tested Application | IP, WB |
| Specificity | This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ELMO1 |
| Antigen Species | Human |
| Immunogen | PKEPSNYDFVYDCN |
| Conjugation | Un-conjugated |
| Alternate Names | Protein ced-12 homolog; ELMO-1; CED12; CED-12; Engulfment and cell motility protein 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8BPU7 Mouse Engulfment and cell motility protein 1 Swiss-port # Q92556 Human Engulfment and cell motility protein 1 |
|---|---|
| Background | This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2011] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 84 kDa |
| PTM | Phosphorylated by HCK. |
Images (2) Click the Picture to Zoom In
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ARG63176 anti-ELMO1 antibody WB image
Western blot: 35 µg of Human frontal cortex (A), Mouse brain (B) and Rat brain (C) lysates (in RIPA buffer) stained with ARG63176 anti-ELMO1 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG63176 anti-ELMO1 antibody WB image
Western blot: 35 µg of Jurkat cell lysate (in RIPA buffer) stained with ARG63176 anti-ELMO1 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
Customer's Feedback
Good
Review for anti-ELMO1 antibody
Application：WB
Sample：Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-ELMO1 antibody
Application：WB
Sample：Mouse brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-ELMO1 antibody
Application：WB
Sample：Jurkat
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
