ARG44460

anti-EIF3B antibody

anti-EIF3B antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes EIF3B
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name EIF3B
Antigen Species Human
Immunogen Human EIF3B recombinant protein
Conjugation Un-conjugated
Alternate Names EIF3B; Eukaryotic Translation Initiation Factor 3 Subunit B; EIF3S9; PRT1; Eukaryotic Translation Initiation Factor 3, Subunit 9 Eta, 116kDa; Eukaryotic Translation Initiation Factor 3 Subunit 9; Protein Synthesis 1; Prt1 Homolog; Eukaryotic Translation Initiation Factor 3, Subunit 9 (Eta, 116kD); Eukaryotic Translation Initiation Factor 3, Subunit B

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/1x10^6
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified with Immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27979 Mouse EIF3B

GeneID: 288516 Rat EIF3B

GeneID: 8662 Human EIF3B

Gene Symbol EIF3B
Gene Full Name Eukaryotic Translation Initiation Factor 3 Subunit B
Background Enables RNA binding activity. Contributes to translation initiation factor activity. Involved in several processes, including IRES-dependent viral translational initiation; translational initiation; and viral translational termination-reinitiation. Located in extracellular exosome. Part of eukaryotic translation initiation factor 3 complex.
Function RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
Cellular Localization Cytoplasm
Calculated MW 92 kDa
PTM Acetylation, Phosphoprotein

Images (6) Click the Picture to Zoom In

  • ARG44460 anti-EIF3B antibody IHC-P image

    Immunohistochemistry: Human colon adenocarcinoma stained with ARG44460 anti-EIF3B antibody at 2 μg/mL dilution.

  • ARG44460 anti-EIF3B antibody ICC/IF image

    Immunofluorescence: SiHa stained with ARG44460 anti-EIF3B antibody at 5 μg/mL dilution.

  • ARG44460 anti-EIF3B antibody WB image

    Western blot: 293T, Jurkat and HeLa stained with ARG44460 anti-EIF3B antibody at 0.5 μg/mL dilution.

  • ARG44460 anti-EIF3B antibody FACS image

    Flow Cytometry: Caco-2 stained with ARG44460 anti-EIF3B antibody at 1 μg/10^6 cells dilution.

  • ARG44460 anti-EIF3B antibody IHC-P image

    Immunohistochemistry: Rat testis stained with ARG44460 anti-EIF3B antibody at 2 μg/mL dilution.

  • ARG44460 anti-EIF3B antibody IHC-P image

    Immunohistochemistry: Mouse testis stained with ARG44460 anti-EIF3B antibody at 2 μg/mL dilution.