ARG44460

anti-EIF3B antibody

anti-EIF3B antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot,Flow cytometry and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes EIF3B
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name EIF3B
Antigen Species Human
Immunogen Human EIF3B recombinant protein
Conjugation Un-conjugated
Alternate Names EIF3B; Eukaryotic Translation Initiation Factor 3 Subunit B; EIF3S9; PRT1; Eukaryotic Translation Initiation Factor 3, Subunit 9 Eta, 116kDa; Eukaryotic Translation Initiation Factor 3 Subunit 9; Protein Synthesis 1; Prt1 Homolog; Eukaryotic Translation Initiation Factor 3, Subunit 9 (Eta, 116kD); Eukaryotic Translation Initiation Factor 3, Subunit B

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/1x10^6
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 27979 Mouse EIF3B

GeneID: 288516 Rat EIF3B

GeneID: 8662 Human EIF3B

Gene Symbol EIF3B
Gene Full Name Eukaryotic Translation Initiation Factor 3 Subunit B
Background Enables RNA binding activity. Contributes to translation initiation factor activity. Involved in several processes, including IRES-dependent viral translational initiation; translational initiation; and viral translational termination-reinitiation. Located in extracellular exosome. Part of eukaryotic translation initiation factor 3 complex.
Function RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
Cellular Localization Cytoplasm
Calculated MW 92 kDa
PTM Acetylation, Phosphoprotein

Images (6) Click the Picture to Zoom In

  • ARG44460 anti-EIF3B antibody IHC-P image

    Immunohistochemistry: Human colon adenocarcinoma stained with ARG44460 anti-EIF3B antibody at 2 μg/mL dilution.

  • ARG44460 anti-EIF3B antibody ICC/IF image

    Immunofluorescence: SiHa stained with ARG44460 anti-EIF3B antibody at 5 μg/mL dilution.

  • ARG44460 anti-EIF3B antibody WB image

    Western blot: 293T, Jurkat and HeLa stained with ARG44460 anti-EIF3B antibody at 0.5 μg/mL dilution.

  • ARG44460 anti-EIF3B antibody FACS image

    Flow Cytometry: Caco-2 stained with ARG44460 anti-EIF3B antibody at 1 μg/10^6 cells dilution.

  • ARG44460 anti-EIF3B antibody IHC-P image

    Immunohistochemistry: Rat testis stained with ARG44460 anti-EIF3B antibody at 2 μg/mL dilution.

  • ARG44460 anti-EIF3B antibody IHC-P image

    Immunohistochemistry: Mouse testis stained with ARG44460 anti-EIF3B antibody at 2 μg/mL dilution.