ARG66067
anti-EGF antibody (Biotin)
anti-EGF antibody (Biotin) for ELISA,Western blot and Mouse
Overview
| Product Description | Biotin-conjugated Goat Polyclonal antibody recognizes EGF |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EGF |
| Antigen Species | Mouse |
| Immunogen | E. coli derived recombinant Mouse EGF. (NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR) |
| Conjugation | Biotin |
| Alternate Names | Urogastrone; Pro-epidermal growth factor; URG; HOMG4; EGF |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Egf |
| Gene Full Name | epidermal growth factor |
| Background | This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
| Function | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro. [UniProt] |
| Calculated MW | 134 kDa |
| PTM | O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). |
Images (2) Click the Picture to Zoom In
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ARG66067 anti-EGF antibody (Biotin) standard curve image
Direct ELISA: ARG66067 anti-EGF antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66067 anti-EGF antibody (Biotin) standard curve image
Sandwich ELISA: ARG66067 anti-EGF antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66066 anti-EGF antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
