ARG57039
anti-EDAR antibody [19E8]
anti-EDAR antibody [19E8] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [19E8] recognizes EDAR |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 19E8 |
| Isotype | IgG2a, kappa |
| Target Name | EDAR |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 27-448 of Human EDAR. |
| Conjugation | Un-conjugated |
| Alternate Names | ECTD10B; Ectodermal dysplasia receptor; DL; ECTD10A; EDA1R; Tumor necrosis factor receptor superfamily member EDAR; ED1R; Downless homolog; Ectodysplasin-A receptor; ED5; HRM1; ED3; EDA3; Anhidrotic ectodysplasin receptor 1; EDA-A1R; EDA-A1 receptor |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UNE0 Human Tumor necrosis factor receptor superfamily member EDAR |
|---|---|
| Gene Symbol | EDAR |
| Gene Full Name | ectodysplasin A receptor |
| Background | This gene encodes a member of the tumor necrosis factor receptor family. The encoded transmembrane protein is a receptor for the soluble ligand ectodysplasin A, and can activate the nuclear factor-kappaB, JNK, and caspase-independent cell death pathways. It is required for the development of hair, teeth, and other ectodermal derivatives. Mutations in this gene result in autosomal dominant and recessive forms of hypohidrotic ectodermal dysplasia. [provided by RefSeq, Jul 2008] |
| Function | Receptor for EDA isoform A1, but not for EDA isoform A2. Mediates the activation of NF-kappa-B and JNK. May promote caspase-independent cell death. [UniProt] |
| Calculated MW | 49 kDa |
Images (2) Click the Picture to Zoom In
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ARG57039 anti-EDAR antibody [19E8] FACS image
Flow Cytometry: HeLa cell line stained with ARG57039 anti-EDAR antibody [19E8] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57039 anti-EDAR antibody [19E8] WB image
Western blot: 1) 50 ng of Recombinant protein, 2) 40 µg of A549 cell lysate, 3) 40 µg of Ramos cell lysate stained with ARG57039 anti-EDAR antibody [19E8] at 1:1000.
