ARG57553

anti-ECHS1 antibody

anti-ECHS1 antibody for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description

Rabbit Polyclonal antibody recognizes ECHS1

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ECHS1
Antigen Species Human
Immunogen Recombinant protein of Human ECHS1.
Conjugation Un-conjugated
Alternate Names Enoyl-CoA hydratase 1; SCEH; Enoyl-CoA hydratase, mitochondrial; EC 4.2.1.17; Short-chain enoyl-CoA hydratase; ECHS1D

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control U87
Observed Size 31 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 140547 Rat ECHS1

GeneID: 1892 Human ECHS1

GeneID: 93747 Mouse ECHS1

Gene Symbol ECHS1
Gene Full Name enoyl CoA hydratase, short chain, 1, mitochondrial
Background The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq, Jul 2008]
Function Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. [UniProt]
Calculated MW 31 kDa

Images (2) Click the Picture to Zoom In

  • ARG57553 anti-ECHS1 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG57553 anti-ECHS1 antibody at 1:100 dilution.

  • ARG57553 anti-ECHS1 antibody WB image

    Western blot: 25 µg of U87 cell lysate stained with ARG57553 anti-ECHS1 antibody at 1:1000 dilution.