ARG52961
anti-Dysferlin antibody
anti-Dysferlin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Dysferlin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Dysferlin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from C-terminus of human dysferlin. |
| Conjugation | Un-conjugated |
| Alternate Names | FER1L1; Dystrophy-associated fer-1-like protein; Fer-1-like protein 1; LGMD2B; MMD1; Dysferlin |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Skeletal Muscle, Human Skeletal Muscle |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Dysferlin is the protein product of the 2p13 gene that is defective in patients with Limb-Girdle Muscular Dystrophy type 2B (LGMD2B) and Miyoshi Myopathy (MM). Dysferlin is normally localized to the muscle plasma membrane. In patients with LGMD2B and MM, immunoreactivity to dysferlin is severely reduced or lost, depending on the type of mutation. |
| Cellular Localization | Cytoplasm, Membrane |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 237 kDa |
Images (2) Click the Picture to Zoom In
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ARG52961 anti-Dysferlin antibody WB image
Western Blot: skeletal muscle stained with ARG52961 anti-Dysferlin antibody
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ARG52961 anti-Dysferlin antibody IHC-P image
Immunohistochemistry: Human Skeletal Muscle stained with ARG52961 anti-Dysferlin antibody
