ARG64515
anti-Dual oxidase 1 antibody
anti-Dual oxidase 1 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Dual oxidase 1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_059130.2 ; NP_787954.1) which are identical; |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Dual oxidase 1 |
| Antigen Species | Human |
| Immunogen | EKFQRSCLHQTVQ |
| Conjugation | Un-conjugated |
| Alternate Names | EC 1.11.1.-; NOXEF1; EC 1.6.3.1; Large NOX 1; Long NOX 1; NADPH thyroid oxidase 1; LNOX1; THOX1; Thyroid oxidase 1; Dual oxidase 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes proteins encoded by this gene and the similar DUOX2 gene. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. This protein generates hydrogen peroxide and thereby plays a role in the activity of thyroid peroxidase, lactoperoxidase, and in lactoperoxidase-mediated antimicrobial defense at mucosal surfaces. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2012] |
| Research Area | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 177 kDa |
| PTM | N-glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG64515 anti-Dual oxidase 1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Thyroid Gland. (Steamed antigen retrieval with Tris/EDTA buffer pH 9) stained with ARG64515 anti-Dual oxidase 1 antibody at 4 µg/ml dilution followed by HRP-staining.
